Cooked my starters

[BrianL]

I put my starters on stir plates inside my fermentation freezer with a brewbelt to keep the temp a nice 71F, but I left the sensor outside the freezer! It was about 95F in there. The yeast fermented out obviously, anyone think I did anything bad/weird to the yeast?

[BrianL]

Yeasts were 001 and 002 by the way. I just caught it and I pitched Monday night, I think it took a while to get that hot, so I'm thinking they're ok.

[BDawg]

Honestly, I'd start over with new vials.
The cells you produced grew up in an environment that is a good 30 or so degrees hotter than what you will be subjecting them to.
The cells that survived and grew essentially self-selected to match that environment.
They will be fuselly and estery, and may flocculate out at the colder temps.

my .02-

[BrianL]

I think that's sound advice, but I'm going to roll with it for the sake of science and laziness.

[snowcapt]

I think that's sound advice, but I'm going to roll with it for the sake of science and laziness.

+1 brotha. Ya never know until you try, right? Cheers to the name of laziness, er, I mean, science.

[BrianL]

Update-

Pitched last night. This morning, nothing happening in either carboy. They're usually going strong at this point. I picked up a couple new vials this morning, racked out 500 mils of wort into flasks and threw them on a stir plate. I just checked the carboys, the 001 finally got going on its own, thin yeast layer on the top. 002, still nothing. I added oxygen to the carboy and pitched the 002.

The 001 was going, but I knew the yeast couldn't be 100% since it took them so long to start in a 1.045 wort. I didn't want to totally overpitch, so I threw in half the starter.

We'll see what happens.

Lesson-if you cook your starters....start over.