Tue Sep 08, 2015 10:56 am
I was able to capture evidence
Re-iterating the experiment description:
I sterilized 5 jars with their caps in a pot of boiling water for 15 minutes and then filled each jar with cooled DME that I made (just like a starter but without adding yeast.). I then poured the DME into each jar and a put one by the sink/washer where I cool my wort and one in the fermentation chamber where they sat open for 2 hours before I capped them. As for the other three, one was capped right away which was the control, one was aerated with a dry but un-sanitized aeration stone, and the last one was put on the stir plate for 24-36 hours.
Notes from Thursday before capping each one:
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Control:
PH: 5.95
Brix: 11
Taste/Aroma: tasted and smelled like DME
Near Washer:
PH: 5.96
Brix: 11
Taste/Aroma: tasted and smelled like DME
Aeration stone:
PH: 5.96
Brix: 11
Taste/Aroma: tasted and smelled like DME
Fermentation Chamber:
PH: 5.96
Brix: 11
Taste/Aroma: tasted and smelled like DME
Stir Plate with Tin foil:
PH: 5.96
Brix: 11
Taste/Aroma: tasted and smelled like DME
Sunday Morning (sept. 6th) I checked each one:
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Control:
PH: 3.64
Brix: 11 (no change)
Taste/Aroma: smelled a little tart, stale bland aroma (very similar to my problem batches), no DME aroma, did not taste.
Notes: PH dropped significantly, but zero brix change.
Near Washer:
PH: 3.64
Brix: 11 (no change)
Taste/Aroma: same as control ^
Notes: PH dropped significantly, but zero brix change.
Aeration stone:
PH: 3.96
Brix: 10 (dropped a whole 1 Brix)
Taste/Aroma: smelled terrible, moldy sour cheesy, sour, tart. Defiantly infected. Did not taste.
Notes: PH dropped significantly, but brix dropped a while 1 brix indicating wild yeast infection.
Fermentation Chamber:
PH: 4.66
Brix: 9.6 (dropped 1.4 Brix)
Taste/Aroma: similar to aeration stone but way more apparent moldy sour cheese, musky. Sour. Did not taste. Def infected.
Notes: PH dropped significantly, but brix dropped a while 1.4 brix indicating wild yeast infection.
24hour Stir Plate with Tin foil:
PH: 3.60
Brix: 11 (no change)
Taste/Aroma: smelled kinda tart, a bit dme aroma, oxizided (which expected). Tasted a couple drops on the tongue. Slightly tart, slight dme taste, not funky, tasted like a finished starter with out yeast character.
Notes: PH dropped significantly, but zero brix change.
Conclusion:
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The fermentation chamber and aeration stone jars both had higher finishing PHs 4.66 and 3.96 respectively. They ALSO had a Brix/gravity change 1.4 and 1 Brix respectively indicating wild yeast. They also exhibited complete signs of infection given the ph, aromas and gravity change they are LEAST likely correlate with the issues of my problem batches and here's why.
1.) The aeration stone was not sanitized or sterilized before aerating the jar and I do that before aerating a batch of fresh wort.
2.) my fermenting beers get a airlock and are not exposed to the open air of the fridge for significant amounts of time.
The stir plate, control, and sink/washer jars both had a PH drop down to 3.65~ but the brix/gravity did NOT change at all. This tells me that this is NOT wild yeast but is some type of lactic acid producing bacteria? that is airborne and it is the same strain that contaminated each of the three jars and what has been contaminating my brewery for the last 6 months. Something is living in my air, and being inoculated in the starter and/or during cooling when the wort hits levels the bacteria can survive in.
* as for the control jar, I'm not sure how that contaminated (it shouldn't have been) but it must have sat out longer then I thought before I capped it.
Last edited by
HotCheese on Wed Dec 09, 2015 10:50 am, edited 2 times in total.