basilhays wrote: 5.5 gal at 1.053OG. Cooled to 50F.
I made a 2L starter 36 hours prior with 2 vials of WLP830 (stir plate). Cooled the starter down to 45F poured off the wort and pitched the slurry.
12 hours later, no activity.
How long does it take before I should see fermentation activity?
In my experience with lagers you should see activity within 12 hrs provided the yeast were well mixed into the wort and the wort was well oxygenated (2 -3 times air saturation). I usually have gas evolution (bubbles in the "air lock") and a partial foam cap at the end of 12 hrs but for sure I have an 0.5 - 0.8 unit drop in pH. A pH drop within a few (6?) hours is usually a reliable sign that the fermentation is off to a good start.
One thing you might want to do is get down so your eye level is close to the surface of the wort and shine a flashlight onto the surface at a sharp angle. This will make it easy to see tiny bubbles breaking the surface. The surface sort of "sparkles" from these well before any foam cap forms.
basilhays wrote: Should I raise the temperture up closer to 55F to encourage activity? If no activity starts, when should I consider pitching additional yeast?
On the assumption that the starter kicked off well (smelled clean, good foam cap, gas laden liquid i.e. vigorously fermented the starter) you should have plenty of yeast. If nothing is happening at the end of 12 - 24 hrs (temp 48 - 50 °F) something else is wrong (under oxygenated, starter not active, low FAN ...). So I'd worry a little - but not too much. It might finally kick off in a day or 2. In the future try to figure out what could be improved. We really do like to have a solid, full blown fermentation going within 24 - 36 hrs.